Cross-Reactive Antibody Responses to Novel H5Nx Influenza Viruses Following Homologous and Heterologous Prime-Boost Vaccination with a Prepandemic Stockpiled A(H5N1) Vaccine in Humans

Publication Type
Journal Article
Year of Publication
2017
Authors
Levine, Min Z; Holiday, Crystal; Liu, Feng; Jefferson, Stacie; Gillis, Eric; Bellamy, Abbie R; Tumpey, Terrence; Katz, Jacqueline M
Secondary
J Infect Dis
Volume
216
Start Page
S555
Pagination
S555-S559
Date Published
09/2017
Keywords
Adjuvants, Immunologic; Adolescent; Adult; Antibodies, Viral; Antibody Formation; Cross Reactions; Dose-Response Relationship, Drug; Hemagglutination Inhibition Tests; Humans; Immunization, Secondary; Influenza A Virus, H5N1 Subtype; Influenza Vaccines; Influenza, Human; Middle Aged; Randomized Controlled Trials as Topic; Young Adult
Abstract

Recently, novel highly pathogenic avian influenza H5Nx viruses (clade 2.3.4.4) caused outbreaks in US poultry. We evaluated the potential of a stockpiled A(H5N1) A/Anhui/1/2005 (clade 2.3.4) vaccine to elicit cross-reactive antibody responses to these emerging viruses. Sera from subjects who received 2 doses of MF59-adjuvanted A/Anhui/1/2005, or 1 dose of MF59-adjuvanted A/Anhui/1/2005 following priming with a clade 1 vaccine were characterized by microneutralization assays and modified hemagglutination inhibition (HI) assays. Only heterologous prime-boost vaccination induced modest cross-reactive HI antibody responses to H5Nx viruses. Heterologous prime-boost may provide a more effective vaccination strategy to broaden the antibody responses to emerging viruses.