DNA vaccine delivered by a needle-free injection device improves potency of priming for antibody and CD8+ T-cell responses after rAd5 boost in a randomized clinical trial.

Publication Type
Journal Article
Year of Publication
2013
Authors
Graham, Barney S; Enama, Mary E; Nason, Martha C; Gordon, Ingelise J; Peel, Sheila A; Ledgerwood, Julie E; Plummer, Sarah A; Mascola, John R; Bailer, Robert T; Roederer, Mario; Koup, Richard A; Nabel, Gary J; VRC 008 Study Team
Secondary
PLoS One
Volume
8
Pagination
e59340
Date Published
2013
Keywords
Adenoviridae; Adolescent; Adult; Antibodies, Viral; CD8-Positive T-Lymphocytes; DNA, Recombinant; Dose-Response Relationship, Immunologic; Female; HIV-1; Humans; Immunity, Cellular; Immunity, Humoral; Immunization, Secondary; Injections; Male; Middle Aged; Peptide Fragments; safety; Vaccination; Vaccines, DNA; Young Adult
Abstract

BACKGROUND: DNA vaccine immunogenicity has been limited by inefficient delivery. Needle-free delivery of DNA using a CO2-powered Biojector® device was compared to delivery by needle and syringe and evaluated for safety and immunogenicity.

METHODS: Forty adults, 18-50 years, were randomly assigned to intramuscular (IM) vaccinations with DNA vaccine, VRC-HIVDNA016-00-VP, (weeks 0, 4, 8) by Biojector® 2000™ or needle and syringe (N/S) and boosted IM at week 24 with VRC-HIVADV014-00-VP (rAd5) with N/S at 10(10) or 10(11) particle units (PU). Equal numbers per assigned schedule had low (≤500) or high (>500) reciprocal titers of preexisting Ad5 neutralizing antibody.

RESULTS: 120 DNA and 39 rAd5 injections were given; 36 subjects completed follow-up research sample collections. IFN-γ ELISpot response rates were 17/19 (89%) for Biojector® and 13/17 (76%) for N/S delivery at Week 28 (4 weeks post rAd5 boost). The magnitude of ELISpot response was about 3-fold higher in Biojector® compared to N/S groups. Similar effects on response rates and magnitude were observed for CD8+, but not CD4+ T-cell responses by ICS. Env-specific antibody responses were about 10-fold higher in Biojector-primed subjects.

CONCLUSIONS: DNA vaccination by Biojector® was well-tolerated and compared to needle injection, primed for greater IFN-γ ELISpot, CD8+ T-cell, and antibody responses after rAd5 boosting.

TRIAL REGISTRATION: ClinicalTrials.gov NCT00109629.